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    Bio-Rad rs 122 2301 dc protein assay bio rad
    Rs 122 2301 Dc Protein Assay Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 16470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rs+122+2301+dc+protein+assay+bio+rad/DC+Protein+Assay+Kit+I/pm39084216-598-68-72
    Average 97 stars, based on 16470 article reviews
    rs 122 2301 dc protein assay bio rad - by Bioz Stars, 2026-09
    97/100 stars

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    Virus:

    Article Title: Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP5K proteintech Cat# 16483-1-AP; RRID: AB_2062052 HSP90 Cell Signaling Cat# 4877; RRID: AB_2233307 UCP1 Invitrogen Cat# PA-124894; RRID: AB_2241459 PERILIPIN1 Cell Signaling Cat# 9349; RRID: AB_10829911 ATP5I Abcam Cat# ab122241; RRID: AB_11127890 GFP Abcam Cat# ab290; RRID: AB_303395 CKMT2 proteintech Cat# 13207-1-AP; RRID: AB_2081188 SERCA2 ATPase antibody [2A7-A1] Abcam Cat# ab2861; RRID: AB_2061425 total oxphos rodent wb antibody Abcam Cat# ab110413; RRID: AB_2629281 Glycerol Kinase (GK) Abcam Cat# ab126599; RRID: AB_11129767 Alexa Fluor 594 anti-rabbit Jackson ImmunoResearch Cat# 711-585-152; RRID: AB_2340621 Alexa Fluor 647 anti-mouse Jackson ImmunoResearch Cat# 715-605-151; RRID: AB_2340863 Alexa Fluor 647 anti-goat ThermoFisher Cat# A21447; RRID: AB_141844 Alexa Fluor 647 anti-rat Jackson ImmunoResearch Cat# 111-605-003; RRID: AB_233807 Chemicals, peptides, and recombinant proteins Hoechst 33342 Cell Signaling Cat# 4082 AAVanced Concentration Reagent BioCat GmbH Cat# AAV110A-1 TransDux MAX Lentivirus Transduction Reagent BioCat GmbH Cat# LV860A-1 RNAiMAX Transfection Reagent Invitrogen Cat# 13778150 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat# I5879 Dexamethasone Sigma-Aldrich Cat# D4902 Indomethacin Sigma-Aldrich Cat# I7378 Insulin Sigma-Aldrich Cat# I9278 Rosiglitazone Adipogen Cat# 71740 Triiodo-L-Thyronine (T3) Sigma-Aldrich Cat# T6397 CL-316,243 Sigma-Aldrich Cat# C5976 Collagen, Type I Sigma-Aldrich Cat# C3867 DMEM, low glucose Lonza Cat# BE12-707F Ham’s F12 medium Lonza Cat# BE12-615F Penicillin-Streptomycin Gibco Cat# 15070063 DMEM, high glucose Gibco Cat# 41965062 Opti-MEM I reduced serum Gibco Cat# 31985062 L-glutamine Gibco Cat# 25030-024 Trizol reagent Invitrogen Cat# 15596026 DNase I (RNase-free) NEB Cat# M0303 Forskolin Sigma-Aldrich Cat# 6886 Tamoxifen Sigma-Aldrich Cat# T5648 D-glucose Sigma-Aldrich Cat# G7021 Oligomycin Adipogen Cat# 11342 Isoproterenol Sigma-Aldrich Cat# I5627 Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 FCCP Sigma-Aldrich Cat# C2920 Rotenone Sigma-Aldrich Cat# R8875 Antimycin A Sigma-Aldrich Cat# A8674 Sodium pyruvate Invitrogen Cat# 11360070 (Continued on next page) Cell Metabolism 36, 1–16.e1–e7, September 3, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Seahorse XF Base Medium Agilent Cat# 102353 ATGL inhibitor Invitrogen Cat#SML1075 HSL inhibitor MedChemExpress Cat# HY-102056 SBI-425 inhibitor Sigma Cat# SML2935-25MG FL C16-Bodipy Invitrogen Cat# D3821 Complete Protease Inhibitors Roche Cat# 05056489001 Halt Phosphatase Inhibitors ThermoFischer Cat# 78426 PEG-it Virus precipitation solution BioCat Cat# LV825A-1-SBI Critical commercial assays High-Capacity cDNA RT kit Applied Biosystems Cat# 4368814 TruSeq RNA library prep kit Illumina Cat# RS-122-2301 DC Protein Assay Bio-Rad Cat# 5000111 XFE96 FluxPak Agilent Cat# 102416-100 Cell Counting Kit 8 Abcam Cat# ab228554 ATP Detection Assay Kit Abcam Cat# ab113849 Chromium Single Cell V3.1 10X Genomics Cat # PN-1000121; PN-1000127; PN-1000213 Deposited data Human and mouse snRNAseq DATA NCBI GEO: GSE184707 Bulk RNAseq data from BAT NCBI GEO: GSE220158 Bulk RNAseq data from VAT/SAT NCBI Available upon request from C.W. and M.B. .. Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl mice GemPharmatech N/A Oligonucleotides qPCR primers Microsynth Listed in STAR Methods siRNAs Microsynth Listed in STAR Methods Recombinant DNA pLenti-MP2 plasmid Addgene Cat# 36097; RRID: Addgene_36097 Atp5k promoter sequences GenScript N/A pAAV-CA Addgene Cat# 69616; RRID: Addgene_69616 Software and algorithms Cellranger 10X Genomics V4.0.0 CellBender Fleming et al.63 V0.2.0 scDblFinder Pierre-Luc Germain64 V1.5.11 Seurat Hao et al.65 V4.0.4 SingleR Aran et al.66 V1.6.1 clusterProfiler Wu et al.67 V4.8.1 SUSHI Hatakeyama et al.68; Qi et al.69 N/A STAR Dobin et al.70 v2.7.4a (Continued on next page) e2 Cell Metabolism 36, 1–16.e1–e7, September 3, 2024

    DC Protein Assay:

    Article Title: Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP5K proteintech Cat# 16483-1-AP; RRID: AB_2062052 HSP90 Cell Signaling Cat# 4877; RRID: AB_2233307 UCP1 Invitrogen Cat# PA-124894; RRID: AB_2241459 PERILIPIN1 Cell Signaling Cat# 9349; RRID: AB_10829911 ATP5I Abcam Cat# ab122241; RRID: AB_11127890 GFP Abcam Cat# ab290; RRID: AB_303395 CKMT2 proteintech Cat# 13207-1-AP; RRID: AB_2081188 SERCA2 ATPase antibody [2A7-A1] Abcam Cat# ab2861; RRID: AB_2061425 total oxphos rodent wb antibody Abcam Cat# ab110413; RRID: AB_2629281 Glycerol Kinase (GK) Abcam Cat# ab126599; RRID: AB_11129767 Alexa Fluor 594 anti-rabbit Jackson ImmunoResearch Cat# 711-585-152; RRID: AB_2340621 Alexa Fluor 647 anti-mouse Jackson ImmunoResearch Cat# 715-605-151; RRID: AB_2340863 Alexa Fluor 647 anti-goat ThermoFisher Cat# A21447; RRID: AB_141844 Alexa Fluor 647 anti-rat Jackson ImmunoResearch Cat# 111-605-003; RRID: AB_233807 Chemicals, peptides, and recombinant proteins Hoechst 33342 Cell Signaling Cat# 4082 AAVanced Concentration Reagent BioCat GmbH Cat# AAV110A-1 TransDux MAX Lentivirus Transduction Reagent BioCat GmbH Cat# LV860A-1 RNAiMAX Transfection Reagent Invitrogen Cat# 13778150 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat# I5879 Dexamethasone Sigma-Aldrich Cat# D4902 Indomethacin Sigma-Aldrich Cat# I7378 Insulin Sigma-Aldrich Cat# I9278 Rosiglitazone Adipogen Cat# 71740 Triiodo-L-Thyronine (T3) Sigma-Aldrich Cat# T6397 CL-316,243 Sigma-Aldrich Cat# C5976 Collagen, Type I Sigma-Aldrich Cat# C3867 DMEM, low glucose Lonza Cat# BE12-707F Ham’s F12 medium Lonza Cat# BE12-615F Penicillin-Streptomycin Gibco Cat# 15070063 DMEM, high glucose Gibco Cat# 41965062 Opti-MEM I reduced serum Gibco Cat# 31985062 L-glutamine Gibco Cat# 25030-024 Trizol reagent Invitrogen Cat# 15596026 DNase I (RNase-free) NEB Cat# M0303 Forskolin Sigma-Aldrich Cat# 6886 Tamoxifen Sigma-Aldrich Cat# T5648 D-glucose Sigma-Aldrich Cat# G7021 Oligomycin Adipogen Cat# 11342 Isoproterenol Sigma-Aldrich Cat# I5627 Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 FCCP Sigma-Aldrich Cat# C2920 Rotenone Sigma-Aldrich Cat# R8875 Antimycin A Sigma-Aldrich Cat# A8674 Sodium pyruvate Invitrogen Cat# 11360070 (Continued on next page) Cell Metabolism 36, 1–16.e1–e7, September 3, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Seahorse XF Base Medium Agilent Cat# 102353 ATGL inhibitor Invitrogen Cat#SML1075 HSL inhibitor MedChemExpress Cat# HY-102056 SBI-425 inhibitor Sigma Cat# SML2935-25MG FL C16-Bodipy Invitrogen Cat# D3821 Complete Protease Inhibitors Roche Cat# 05056489001 Halt Phosphatase Inhibitors ThermoFischer Cat# 78426 PEG-it Virus precipitation solution BioCat Cat# LV825A-1-SBI Critical commercial assays High-Capacity cDNA RT kit Applied Biosystems Cat# 4368814 TruSeq RNA library prep kit Illumina Cat# RS-122-2301 DC Protein Assay Bio-Rad Cat# 5000111 XFE96 FluxPak Agilent Cat# 102416-100 Cell Counting Kit 8 Abcam Cat# ab228554 ATP Detection Assay Kit Abcam Cat# ab113849 Chromium Single Cell V3.1 10X Genomics Cat # PN-1000121; PN-1000127; PN-1000213 Deposited data Human and mouse snRNAseq DATA NCBI GEO: GSE184707 Bulk RNAseq data from BAT NCBI GEO: GSE220158 Bulk RNAseq data from VAT/SAT NCBI Available upon request from C.W. and M.B. .. Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl mice GemPharmatech N/A Oligonucleotides qPCR primers Microsynth Listed in STAR Methods siRNAs Microsynth Listed in STAR Methods Recombinant DNA pLenti-MP2 plasmid Addgene Cat# 36097; RRID: Addgene_36097 Atp5k promoter sequences GenScript N/A pAAV-CA Addgene Cat# 69616; RRID: Addgene_69616 Software and algorithms Cellranger 10X Genomics V4.0.0 CellBender Fleming et al.63 V0.2.0 scDblFinder Pierre-Luc Germain64 V1.5.11 Seurat Hao et al.65 V4.0.4 SingleR Aran et al.66 V1.6.1 clusterProfiler Wu et al.67 V4.8.1 SUSHI Hatakeyama et al.68; Qi et al.69 N/A STAR Dobin et al.70 v2.7.4a (Continued on next page) e2 Cell Metabolism 36, 1–16.e1–e7, September 3, 2024

    Article Title: Inhibition of Mevalonate Pathway Prevents Adipocyte Browning in Mice and Men by Affecting Protein Prenylation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies UCP1 Thermo Fisher Cat# PA1-24894; RRID: AB_2241459 HSP90 Cell Signaling Cat# 4877; RRID: AB_2233307 g-TUBULIN Sigma-Aldrich Cat# T-5326; RRID: AB_532292 HSL Cell Signaling Cat# 4107; RRID: AB_2296900 phospho-HSL (Ser660) Cell Signaling Cat# 4126; RRID: AB_490997 AKT Cell Signaling Cat# 9272; RRID: AB_329827 phospho-AKT (Thr308) Cell Signaling Cat# 13038; RRID: AB_2629447 CREB Cell Signaling Cat# 9197; RRID: AB_331277 phospho-CREB (Ser133) Cell Signaling Cat# 9198; RRID: AB_2561044 PGGT1B Abcam Cat# ab122122; RRID: AB_11129941 RABGGTB Abcam Cat# ab187717; RRID:AB_2751007 GAPDH Cell Signaling Cat# 5174; RRID: AB_10622025 cleaved Caspase 3 (Asp175) Cell Signaling Cat# 9661; RRID: AB_2341188 RHOA Cell Signaling Cat# 2117; RRID: AB_10693922 RAC1/2/3 Cell Signaling Cat# 2465; RRID: AB_10695732 CDC42 Cell Signaling Cat# 2466; RRID: AB_2078082 RAP1A/RAP1B Cell Signaling Cat# 4938; RRID: AB_2177112 Na+/K+ ATPase Abcam Cat# ab76020; RRID: AB_1310695 HMGCS2 Abcam Cat# ab137043; RRID: AB_2749817 YAP1/TAZ Cell Signaling Cat# 8418; RRID: AB_10950494 PPARg Cell Signaling Cat# 2443; RRID: AB_10694772 HMGCR Abcam Cat# ab174830; RRID: AB_2749818 anti-mouse HRP secondary Millipore Cat# 401253; RRID: AB_437779 anti-rabbit HRP secondary Millipore Cat# 401393; RRID: AB_10683386 Streptavidin-HRP Cell Signaling Cat# 3999; RRID: AB_10830897 Chemicals, Peptides, and Recombinant Proteins Hoechst 33342 Cell Signaling Cat# 4082 Bodipy 493/503 Invitrogen Cat# D3922 Syto60 Invitrogen Cat# S11342 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat# I5879 Dexamethasone Sigma-Aldrich Cat# D4902 Indomethacin Sigma-Aldrich Cat# I7378 Insulin Sigma-Aldrich Cat# I9278 Rosiglitazone Adipogen Cat# 71740 Triiodo-L-Thyronine (T3) Sigma-Aldrich Cat# T6397 CL-316,243 Sigma-Aldrich Cat# C5976 Collagen, Type I Sigma-Aldrich Cat# C3867 DMEM, low glucose Lonza Cat# BE12-707F Ham’s F12 medium Lonza Cat# BE12-615F Penicillin-Streptomycin Gibco Cat# 15070063 DMEM, high glucose Gibco Cat# 41965062 L-glutamine Gibco Cat# 25030-024 Trizol reagent Invitrogen Cat# 15596026 DNase I (RNase-free) NEB Cat# M0303 Simvastatin Sigma-Aldrich Cat# S6196 (Continued on next page) Cell Metabolism 29, 1–16.e1–e8, April 2, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Fluvastatin Sigma-Aldrich Cat# SML0038 Atorvastatin Sigma-Aldrich Cat# PZ0001 Rosuvastatin Sigma-Aldrich Cat# SML1264 Pravastatin Sigma-Aldrich Cat# P4498 Cerivastatin Sigma-Aldrich Cat# SML0005 GGTI-298 Sigma-Aldrich Cat# G5169 FTI-277 Sigma-Aldrich Cat# F9803 Farnesyl pyrophosphate Sigma-Aldrich Cat# F6892 Farnesol Abcam Cat# ab142428 Geranylgeranyl pyrophosphate Sigma-Aldrich Cat# G6025 Geranylgeraniol Abcam Cat# ab142436 Gallein Santa Cruz Cat# sc-202631 Rho inhibitor, Rhosin Calbiochem Cat# 555460 Squalene Sigma-Aldrich Cat# S3626 Coenzyme Q9 Sigma-Aldrich Cat# 27597 Coenzyme Q10 Sigma-Aldrich Cat# C9538 Tamoxifen Sigma-Aldrich Cat# T5648 4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 D-glucose Sigma-Aldrich Cat# G7021 Oligomycin Adipogen Cat# 11342 Isoproterenol Sigma-Aldrich Cat# I5627 Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 FCCP Sigma-Aldrich Cat# C2920 Rotenone Sigma-Aldrich Cat# R8875 Antimycin A Sigma-Aldrich Cat# A8674 Sodium pyruvate Invitrogen Cat# 11360070 Seahorse XF Base Medium Agilent Cat# 102353 Complete Protease Inhibitors Roche Cat# 05056489001 Halt Phosphatase Inhibitors ThermoFischer Cat# 78426 Click-IT Geranylgeranyl Alcohol, Azide Invitrogen Cat# C10249 Biotin Alkyne Invitrogen Cat# B10185 Phalloidin-iFluor 488 Reagent Abcam Cat# ab176753 Critical Commercial Assays High Capacity cDNA RT kit Applied Biosystems Cat# 4368814 TruSeq RNA library prep kit Illumina Cat# RS-122-2301 DC Protein Assay Bio-Rad Cat# 5000111 Click-IT Protein Reaction Kit Invitrogen Cat# C10276 XFE96 FluxPak Agilent Cat# 102416-100 Glycerol reagent Sigma-Aldrich Cat# F6428 QBT fatty acid uptake assay kit Molecular Devices Cat# R6132 Luciferase reporter system Promega Cat# E1501 Autokit Total Ketone Bodies R1 Set Wako Chemicals Cat# 415-73301 Autokit Total Ketone Bodies R2 Set Wako Chemicals Cat# 413-73601 Deposited Data RNA sequencing data for clinical transcriptome study European Nucleotide Archive PRJEB23275 Experimental Models: Cell Lines immortalized brown adipocytes Christian Wolfrum Klein et al., 2002 hMADS cells Ez-Zoubir Amri Elabd et al., 2009 (Continued on next page) e2 Cell Metabolism 29, 1–16.e1–e8, April 2, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Organisms/Strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 Ucp1-CreERT2 x LoxP-Red mice Christian Wolfrum Rosenwald et al., 2013 Pggt1bfl/fl mice Martin Bergo Sjogren et al., 2007 Adip-CreERT2 mice Christian Wolfrum Rosenwald et al., 2013 Oligonucleotides qPCR primers Microsynth Listed in Table S4 siRNAs Microsynth Listed in Table S3 Software and Algorithms Harmony - Operetta software Perkin Elmer version 3.5 ImageJ NIH, USA version 1.50b Phenomaster software TSE systems version 5.6.5 Wave - XF96 software Agilent version 2.3.0.19 ImageQuant LAS 4000 GE Healthcare version 1.1 GraphPad Prism 7 GraphPad software version 7.03 ViiA7 software Applied Biosystems version 1.2.3 Gen5 BioTek version 1.10

    Cell Counting:

    Article Title: Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP5K proteintech Cat# 16483-1-AP; RRID: AB_2062052 HSP90 Cell Signaling Cat# 4877; RRID: AB_2233307 UCP1 Invitrogen Cat# PA-124894; RRID: AB_2241459 PERILIPIN1 Cell Signaling Cat# 9349; RRID: AB_10829911 ATP5I Abcam Cat# ab122241; RRID: AB_11127890 GFP Abcam Cat# ab290; RRID: AB_303395 CKMT2 proteintech Cat# 13207-1-AP; RRID: AB_2081188 SERCA2 ATPase antibody [2A7-A1] Abcam Cat# ab2861; RRID: AB_2061425 total oxphos rodent wb antibody Abcam Cat# ab110413; RRID: AB_2629281 Glycerol Kinase (GK) Abcam Cat# ab126599; RRID: AB_11129767 Alexa Fluor 594 anti-rabbit Jackson ImmunoResearch Cat# 711-585-152; RRID: AB_2340621 Alexa Fluor 647 anti-mouse Jackson ImmunoResearch Cat# 715-605-151; RRID: AB_2340863 Alexa Fluor 647 anti-goat ThermoFisher Cat# A21447; RRID: AB_141844 Alexa Fluor 647 anti-rat Jackson ImmunoResearch Cat# 111-605-003; RRID: AB_233807 Chemicals, peptides, and recombinant proteins Hoechst 33342 Cell Signaling Cat# 4082 AAVanced Concentration Reagent BioCat GmbH Cat# AAV110A-1 TransDux MAX Lentivirus Transduction Reagent BioCat GmbH Cat# LV860A-1 RNAiMAX Transfection Reagent Invitrogen Cat# 13778150 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat# I5879 Dexamethasone Sigma-Aldrich Cat# D4902 Indomethacin Sigma-Aldrich Cat# I7378 Insulin Sigma-Aldrich Cat# I9278 Rosiglitazone Adipogen Cat# 71740 Triiodo-L-Thyronine (T3) Sigma-Aldrich Cat# T6397 CL-316,243 Sigma-Aldrich Cat# C5976 Collagen, Type I Sigma-Aldrich Cat# C3867 DMEM, low glucose Lonza Cat# BE12-707F Ham’s F12 medium Lonza Cat# BE12-615F Penicillin-Streptomycin Gibco Cat# 15070063 DMEM, high glucose Gibco Cat# 41965062 Opti-MEM I reduced serum Gibco Cat# 31985062 L-glutamine Gibco Cat# 25030-024 Trizol reagent Invitrogen Cat# 15596026 DNase I (RNase-free) NEB Cat# M0303 Forskolin Sigma-Aldrich Cat# 6886 Tamoxifen Sigma-Aldrich Cat# T5648 D-glucose Sigma-Aldrich Cat# G7021 Oligomycin Adipogen Cat# 11342 Isoproterenol Sigma-Aldrich Cat# I5627 Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 FCCP Sigma-Aldrich Cat# C2920 Rotenone Sigma-Aldrich Cat# R8875 Antimycin A Sigma-Aldrich Cat# A8674 Sodium pyruvate Invitrogen Cat# 11360070 (Continued on next page) Cell Metabolism 36, 1–16.e1–e7, September 3, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Seahorse XF Base Medium Agilent Cat# 102353 ATGL inhibitor Invitrogen Cat#SML1075 HSL inhibitor MedChemExpress Cat# HY-102056 SBI-425 inhibitor Sigma Cat# SML2935-25MG FL C16-Bodipy Invitrogen Cat# D3821 Complete Protease Inhibitors Roche Cat# 05056489001 Halt Phosphatase Inhibitors ThermoFischer Cat# 78426 PEG-it Virus precipitation solution BioCat Cat# LV825A-1-SBI Critical commercial assays High-Capacity cDNA RT kit Applied Biosystems Cat# 4368814 TruSeq RNA library prep kit Illumina Cat# RS-122-2301 DC Protein Assay Bio-Rad Cat# 5000111 XFE96 FluxPak Agilent Cat# 102416-100 Cell Counting Kit 8 Abcam Cat# ab228554 ATP Detection Assay Kit Abcam Cat# ab113849 Chromium Single Cell V3.1 10X Genomics Cat # PN-1000121; PN-1000127; PN-1000213 Deposited data Human and mouse snRNAseq DATA NCBI GEO: GSE184707 Bulk RNAseq data from BAT NCBI GEO: GSE220158 Bulk RNAseq data from VAT/SAT NCBI Available upon request from C.W. and M.B. .. Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl mice GemPharmatech N/A Oligonucleotides qPCR primers Microsynth Listed in STAR Methods siRNAs Microsynth Listed in STAR Methods Recombinant DNA pLenti-MP2 plasmid Addgene Cat# 36097; RRID: Addgene_36097 Atp5k promoter sequences GenScript N/A pAAV-CA Addgene Cat# 69616; RRID: Addgene_69616 Software and algorithms Cellranger 10X Genomics V4.0.0 CellBender Fleming et al.63 V0.2.0 scDblFinder Pierre-Luc Germain64 V1.5.11 Seurat Hao et al.65 V4.0.4 SingleR Aran et al.66 V1.6.1 clusterProfiler Wu et al.67 V4.8.1 SUSHI Hatakeyama et al.68; Qi et al.69 N/A STAR Dobin et al.70 v2.7.4a (Continued on next page) e2 Cell Metabolism 36, 1–16.e1–e7, September 3, 2024

    Detection Assay:

    Article Title: Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP5K proteintech Cat# 16483-1-AP; RRID: AB_2062052 HSP90 Cell Signaling Cat# 4877; RRID: AB_2233307 UCP1 Invitrogen Cat# PA-124894; RRID: AB_2241459 PERILIPIN1 Cell Signaling Cat# 9349; RRID: AB_10829911 ATP5I Abcam Cat# ab122241; RRID: AB_11127890 GFP Abcam Cat# ab290; RRID: AB_303395 CKMT2 proteintech Cat# 13207-1-AP; RRID: AB_2081188 SERCA2 ATPase antibody [2A7-A1] Abcam Cat# ab2861; RRID: AB_2061425 total oxphos rodent wb antibody Abcam Cat# ab110413; RRID: AB_2629281 Glycerol Kinase (GK) Abcam Cat# ab126599; RRID: AB_11129767 Alexa Fluor 594 anti-rabbit Jackson ImmunoResearch Cat# 711-585-152; RRID: AB_2340621 Alexa Fluor 647 anti-mouse Jackson ImmunoResearch Cat# 715-605-151; RRID: AB_2340863 Alexa Fluor 647 anti-goat ThermoFisher Cat# A21447; RRID: AB_141844 Alexa Fluor 647 anti-rat Jackson ImmunoResearch Cat# 111-605-003; RRID: AB_233807 Chemicals, peptides, and recombinant proteins Hoechst 33342 Cell Signaling Cat# 4082 AAVanced Concentration Reagent BioCat GmbH Cat# AAV110A-1 TransDux MAX Lentivirus Transduction Reagent BioCat GmbH Cat# LV860A-1 RNAiMAX Transfection Reagent Invitrogen Cat# 13778150 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat# I5879 Dexamethasone Sigma-Aldrich Cat# D4902 Indomethacin Sigma-Aldrich Cat# I7378 Insulin Sigma-Aldrich Cat# I9278 Rosiglitazone Adipogen Cat# 71740 Triiodo-L-Thyronine (T3) Sigma-Aldrich Cat# T6397 CL-316,243 Sigma-Aldrich Cat# C5976 Collagen, Type I Sigma-Aldrich Cat# C3867 DMEM, low glucose Lonza Cat# BE12-707F Ham’s F12 medium Lonza Cat# BE12-615F Penicillin-Streptomycin Gibco Cat# 15070063 DMEM, high glucose Gibco Cat# 41965062 Opti-MEM I reduced serum Gibco Cat# 31985062 L-glutamine Gibco Cat# 25030-024 Trizol reagent Invitrogen Cat# 15596026 DNase I (RNase-free) NEB Cat# M0303 Forskolin Sigma-Aldrich Cat# 6886 Tamoxifen Sigma-Aldrich Cat# T5648 D-glucose Sigma-Aldrich Cat# G7021 Oligomycin Adipogen Cat# 11342 Isoproterenol Sigma-Aldrich Cat# I5627 Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 FCCP Sigma-Aldrich Cat# C2920 Rotenone Sigma-Aldrich Cat# R8875 Antimycin A Sigma-Aldrich Cat# A8674 Sodium pyruvate Invitrogen Cat# 11360070 (Continued on next page) Cell Metabolism 36, 1–16.e1–e7, September 3, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Seahorse XF Base Medium Agilent Cat# 102353 ATGL inhibitor Invitrogen Cat#SML1075 HSL inhibitor MedChemExpress Cat# HY-102056 SBI-425 inhibitor Sigma Cat# SML2935-25MG FL C16-Bodipy Invitrogen Cat# D3821 Complete Protease Inhibitors Roche Cat# 05056489001 Halt Phosphatase Inhibitors ThermoFischer Cat# 78426 PEG-it Virus precipitation solution BioCat Cat# LV825A-1-SBI Critical commercial assays High-Capacity cDNA RT kit Applied Biosystems Cat# 4368814 TruSeq RNA library prep kit Illumina Cat# RS-122-2301 DC Protein Assay Bio-Rad Cat# 5000111 XFE96 FluxPak Agilent Cat# 102416-100 Cell Counting Kit 8 Abcam Cat# ab228554 ATP Detection Assay Kit Abcam Cat# ab113849 Chromium Single Cell V3.1 10X Genomics Cat # PN-1000121; PN-1000127; PN-1000213 Deposited data Human and mouse snRNAseq DATA NCBI GEO: GSE184707 Bulk RNAseq data from BAT NCBI GEO: GSE220158 Bulk RNAseq data from VAT/SAT NCBI Available upon request from C.W. and M.B. .. Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl mice GemPharmatech N/A Oligonucleotides qPCR primers Microsynth Listed in STAR Methods siRNAs Microsynth Listed in STAR Methods Recombinant DNA pLenti-MP2 plasmid Addgene Cat# 36097; RRID: Addgene_36097 Atp5k promoter sequences GenScript N/A pAAV-CA Addgene Cat# 69616; RRID: Addgene_69616 Software and algorithms Cellranger 10X Genomics V4.0.0 CellBender Fleming et al.63 V0.2.0 scDblFinder Pierre-Luc Germain64 V1.5.11 Seurat Hao et al.65 V4.0.4 SingleR Aran et al.66 V1.6.1 clusterProfiler Wu et al.67 V4.8.1 SUSHI Hatakeyama et al.68; Qi et al.69 N/A STAR Dobin et al.70 v2.7.4a (Continued on next page) e2 Cell Metabolism 36, 1–16.e1–e7, September 3, 2024

    Single Cell:

    Article Title: Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP5K proteintech Cat# 16483-1-AP; RRID: AB_2062052 HSP90 Cell Signaling Cat# 4877; RRID: AB_2233307 UCP1 Invitrogen Cat# PA-124894; RRID: AB_2241459 PERILIPIN1 Cell Signaling Cat# 9349; RRID: AB_10829911 ATP5I Abcam Cat# ab122241; RRID: AB_11127890 GFP Abcam Cat# ab290; RRID: AB_303395 CKMT2 proteintech Cat# 13207-1-AP; RRID: AB_2081188 SERCA2 ATPase antibody [2A7-A1] Abcam Cat# ab2861; RRID: AB_2061425 total oxphos rodent wb antibody Abcam Cat# ab110413; RRID: AB_2629281 Glycerol Kinase (GK) Abcam Cat# ab126599; RRID: AB_11129767 Alexa Fluor 594 anti-rabbit Jackson ImmunoResearch Cat# 711-585-152; RRID: AB_2340621 Alexa Fluor 647 anti-mouse Jackson ImmunoResearch Cat# 715-605-151; RRID: AB_2340863 Alexa Fluor 647 anti-goat ThermoFisher Cat# A21447; RRID: AB_141844 Alexa Fluor 647 anti-rat Jackson ImmunoResearch Cat# 111-605-003; RRID: AB_233807 Chemicals, peptides, and recombinant proteins Hoechst 33342 Cell Signaling Cat# 4082 AAVanced Concentration Reagent BioCat GmbH Cat# AAV110A-1 TransDux MAX Lentivirus Transduction Reagent BioCat GmbH Cat# LV860A-1 RNAiMAX Transfection Reagent Invitrogen Cat# 13778150 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat# I5879 Dexamethasone Sigma-Aldrich Cat# D4902 Indomethacin Sigma-Aldrich Cat# I7378 Insulin Sigma-Aldrich Cat# I9278 Rosiglitazone Adipogen Cat# 71740 Triiodo-L-Thyronine (T3) Sigma-Aldrich Cat# T6397 CL-316,243 Sigma-Aldrich Cat# C5976 Collagen, Type I Sigma-Aldrich Cat# C3867 DMEM, low glucose Lonza Cat# BE12-707F Ham’s F12 medium Lonza Cat# BE12-615F Penicillin-Streptomycin Gibco Cat# 15070063 DMEM, high glucose Gibco Cat# 41965062 Opti-MEM I reduced serum Gibco Cat# 31985062 L-glutamine Gibco Cat# 25030-024 Trizol reagent Invitrogen Cat# 15596026 DNase I (RNase-free) NEB Cat# M0303 Forskolin Sigma-Aldrich Cat# 6886 Tamoxifen Sigma-Aldrich Cat# T5648 D-glucose Sigma-Aldrich Cat# G7021 Oligomycin Adipogen Cat# 11342 Isoproterenol Sigma-Aldrich Cat# I5627 Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 FCCP Sigma-Aldrich Cat# C2920 Rotenone Sigma-Aldrich Cat# R8875 Antimycin A Sigma-Aldrich Cat# A8674 Sodium pyruvate Invitrogen Cat# 11360070 (Continued on next page) Cell Metabolism 36, 1–16.e1–e7, September 3, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Seahorse XF Base Medium Agilent Cat# 102353 ATGL inhibitor Invitrogen Cat#SML1075 HSL inhibitor MedChemExpress Cat# HY-102056 SBI-425 inhibitor Sigma Cat# SML2935-25MG FL C16-Bodipy Invitrogen Cat# D3821 Complete Protease Inhibitors Roche Cat# 05056489001 Halt Phosphatase Inhibitors ThermoFischer Cat# 78426 PEG-it Virus precipitation solution BioCat Cat# LV825A-1-SBI Critical commercial assays High-Capacity cDNA RT kit Applied Biosystems Cat# 4368814 TruSeq RNA library prep kit Illumina Cat# RS-122-2301 DC Protein Assay Bio-Rad Cat# 5000111 XFE96 FluxPak Agilent Cat# 102416-100 Cell Counting Kit 8 Abcam Cat# ab228554 ATP Detection Assay Kit Abcam Cat# ab113849 Chromium Single Cell V3.1 10X Genomics Cat # PN-1000121; PN-1000127; PN-1000213 Deposited data Human and mouse snRNAseq DATA NCBI GEO: GSE184707 Bulk RNAseq data from BAT NCBI GEO: GSE220158 Bulk RNAseq data from VAT/SAT NCBI Available upon request from C.W. and M.B. .. Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl mice GemPharmatech N/A Oligonucleotides qPCR primers Microsynth Listed in STAR Methods siRNAs Microsynth Listed in STAR Methods Recombinant DNA pLenti-MP2 plasmid Addgene Cat# 36097; RRID: Addgene_36097 Atp5k promoter sequences GenScript N/A pAAV-CA Addgene Cat# 69616; RRID: Addgene_69616 Software and algorithms Cellranger 10X Genomics V4.0.0 CellBender Fleming et al.63 V0.2.0 scDblFinder Pierre-Luc Germain64 V1.5.11 Seurat Hao et al.65 V4.0.4 SingleR Aran et al.66 V1.6.1 clusterProfiler Wu et al.67 V4.8.1 SUSHI Hatakeyama et al.68; Qi et al.69 N/A STAR Dobin et al.70 v2.7.4a (Continued on next page) e2 Cell Metabolism 36, 1–16.e1–e7, September 3, 2024

    RNA sequencing:

    Article Title: Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP5K proteintech Cat# 16483-1-AP; RRID: AB_2062052 HSP90 Cell Signaling Cat# 4877; RRID: AB_2233307 UCP1 Invitrogen Cat# PA-124894; RRID: AB_2241459 PERILIPIN1 Cell Signaling Cat# 9349; RRID: AB_10829911 ATP5I Abcam Cat# ab122241; RRID: AB_11127890 GFP Abcam Cat# ab290; RRID: AB_303395 CKMT2 proteintech Cat# 13207-1-AP; RRID: AB_2081188 SERCA2 ATPase antibody [2A7-A1] Abcam Cat# ab2861; RRID: AB_2061425 total oxphos rodent wb antibody Abcam Cat# ab110413; RRID: AB_2629281 Glycerol Kinase (GK) Abcam Cat# ab126599; RRID: AB_11129767 Alexa Fluor 594 anti-rabbit Jackson ImmunoResearch Cat# 711-585-152; RRID: AB_2340621 Alexa Fluor 647 anti-mouse Jackson ImmunoResearch Cat# 715-605-151; RRID: AB_2340863 Alexa Fluor 647 anti-goat ThermoFisher Cat# A21447; RRID: AB_141844 Alexa Fluor 647 anti-rat Jackson ImmunoResearch Cat# 111-605-003; RRID: AB_233807 Chemicals, peptides, and recombinant proteins Hoechst 33342 Cell Signaling Cat# 4082 AAVanced Concentration Reagent BioCat GmbH Cat# AAV110A-1 TransDux MAX Lentivirus Transduction Reagent BioCat GmbH Cat# LV860A-1 RNAiMAX Transfection Reagent Invitrogen Cat# 13778150 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat# I5879 Dexamethasone Sigma-Aldrich Cat# D4902 Indomethacin Sigma-Aldrich Cat# I7378 Insulin Sigma-Aldrich Cat# I9278 Rosiglitazone Adipogen Cat# 71740 Triiodo-L-Thyronine (T3) Sigma-Aldrich Cat# T6397 CL-316,243 Sigma-Aldrich Cat# C5976 Collagen, Type I Sigma-Aldrich Cat# C3867 DMEM, low glucose Lonza Cat# BE12-707F Ham’s F12 medium Lonza Cat# BE12-615F Penicillin-Streptomycin Gibco Cat# 15070063 DMEM, high glucose Gibco Cat# 41965062 Opti-MEM I reduced serum Gibco Cat# 31985062 L-glutamine Gibco Cat# 25030-024 Trizol reagent Invitrogen Cat# 15596026 DNase I (RNase-free) NEB Cat# M0303 Forskolin Sigma-Aldrich Cat# 6886 Tamoxifen Sigma-Aldrich Cat# T5648 D-glucose Sigma-Aldrich Cat# G7021 Oligomycin Adipogen Cat# 11342 Isoproterenol Sigma-Aldrich Cat# I5627 Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 FCCP Sigma-Aldrich Cat# C2920 Rotenone Sigma-Aldrich Cat# R8875 Antimycin A Sigma-Aldrich Cat# A8674 Sodium pyruvate Invitrogen Cat# 11360070 (Continued on next page) Cell Metabolism 36, 1–16.e1–e7, September 3, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Seahorse XF Base Medium Agilent Cat# 102353 ATGL inhibitor Invitrogen Cat#SML1075 HSL inhibitor MedChemExpress Cat# HY-102056 SBI-425 inhibitor Sigma Cat# SML2935-25MG FL C16-Bodipy Invitrogen Cat# D3821 Complete Protease Inhibitors Roche Cat# 05056489001 Halt Phosphatase Inhibitors ThermoFischer Cat# 78426 PEG-it Virus precipitation solution BioCat Cat# LV825A-1-SBI Critical commercial assays High-Capacity cDNA RT kit Applied Biosystems Cat# 4368814 TruSeq RNA library prep kit Illumina Cat# RS-122-2301 DC Protein Assay Bio-Rad Cat# 5000111 XFE96 FluxPak Agilent Cat# 102416-100 Cell Counting Kit 8 Abcam Cat# ab228554 ATP Detection Assay Kit Abcam Cat# ab113849 Chromium Single Cell V3.1 10X Genomics Cat # PN-1000121; PN-1000127; PN-1000213 Deposited data Human and mouse snRNAseq DATA NCBI GEO: GSE184707 Bulk RNAseq data from BAT NCBI GEO: GSE220158 Bulk RNAseq data from VAT/SAT NCBI Available upon request from C.W. and M.B. .. Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl mice GemPharmatech N/A Oligonucleotides qPCR primers Microsynth Listed in STAR Methods siRNAs Microsynth Listed in STAR Methods Recombinant DNA pLenti-MP2 plasmid Addgene Cat# 36097; RRID: Addgene_36097 Atp5k promoter sequences GenScript N/A pAAV-CA Addgene Cat# 69616; RRID: Addgene_69616 Software and algorithms Cellranger 10X Genomics V4.0.0 CellBender Fleming et al.63 V0.2.0 scDblFinder Pierre-Luc Germain64 V1.5.11 Seurat Hao et al.65 V4.0.4 SingleR Aran et al.66 V1.6.1 clusterProfiler Wu et al.67 V4.8.1 SUSHI Hatakeyama et al.68; Qi et al.69 N/A STAR Dobin et al.70 v2.7.4a (Continued on next page) e2 Cell Metabolism 36, 1–16.e1–e7, September 3, 2024

    Luciferase:

    Article Title: Inhibition of Mevalonate Pathway Prevents Adipocyte Browning in Mice and Men by Affecting Protein Prenylation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies UCP1 Thermo Fisher Cat# PA1-24894; RRID: AB_2241459 HSP90 Cell Signaling Cat# 4877; RRID: AB_2233307 g-TUBULIN Sigma-Aldrich Cat# T-5326; RRID: AB_532292 HSL Cell Signaling Cat# 4107; RRID: AB_2296900 phospho-HSL (Ser660) Cell Signaling Cat# 4126; RRID: AB_490997 AKT Cell Signaling Cat# 9272; RRID: AB_329827 phospho-AKT (Thr308) Cell Signaling Cat# 13038; RRID: AB_2629447 CREB Cell Signaling Cat# 9197; RRID: AB_331277 phospho-CREB (Ser133) Cell Signaling Cat# 9198; RRID: AB_2561044 PGGT1B Abcam Cat# ab122122; RRID: AB_11129941 RABGGTB Abcam Cat# ab187717; RRID:AB_2751007 GAPDH Cell Signaling Cat# 5174; RRID: AB_10622025 cleaved Caspase 3 (Asp175) Cell Signaling Cat# 9661; RRID: AB_2341188 RHOA Cell Signaling Cat# 2117; RRID: AB_10693922 RAC1/2/3 Cell Signaling Cat# 2465; RRID: AB_10695732 CDC42 Cell Signaling Cat# 2466; RRID: AB_2078082 RAP1A/RAP1B Cell Signaling Cat# 4938; RRID: AB_2177112 Na+/K+ ATPase Abcam Cat# ab76020; RRID: AB_1310695 HMGCS2 Abcam Cat# ab137043; RRID: AB_2749817 YAP1/TAZ Cell Signaling Cat# 8418; RRID: AB_10950494 PPARg Cell Signaling Cat# 2443; RRID: AB_10694772 HMGCR Abcam Cat# ab174830; RRID: AB_2749818 anti-mouse HRP secondary Millipore Cat# 401253; RRID: AB_437779 anti-rabbit HRP secondary Millipore Cat# 401393; RRID: AB_10683386 Streptavidin-HRP Cell Signaling Cat# 3999; RRID: AB_10830897 Chemicals, Peptides, and Recombinant Proteins Hoechst 33342 Cell Signaling Cat# 4082 Bodipy 493/503 Invitrogen Cat# D3922 Syto60 Invitrogen Cat# S11342 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat# I5879 Dexamethasone Sigma-Aldrich Cat# D4902 Indomethacin Sigma-Aldrich Cat# I7378 Insulin Sigma-Aldrich Cat# I9278 Rosiglitazone Adipogen Cat# 71740 Triiodo-L-Thyronine (T3) Sigma-Aldrich Cat# T6397 CL-316,243 Sigma-Aldrich Cat# C5976 Collagen, Type I Sigma-Aldrich Cat# C3867 DMEM, low glucose Lonza Cat# BE12-707F Ham’s F12 medium Lonza Cat# BE12-615F Penicillin-Streptomycin Gibco Cat# 15070063 DMEM, high glucose Gibco Cat# 41965062 L-glutamine Gibco Cat# 25030-024 Trizol reagent Invitrogen Cat# 15596026 DNase I (RNase-free) NEB Cat# M0303 Simvastatin Sigma-Aldrich Cat# S6196 (Continued on next page) Cell Metabolism 29, 1–16.e1–e8, April 2, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Fluvastatin Sigma-Aldrich Cat# SML0038 Atorvastatin Sigma-Aldrich Cat# PZ0001 Rosuvastatin Sigma-Aldrich Cat# SML1264 Pravastatin Sigma-Aldrich Cat# P4498 Cerivastatin Sigma-Aldrich Cat# SML0005 GGTI-298 Sigma-Aldrich Cat# G5169 FTI-277 Sigma-Aldrich Cat# F9803 Farnesyl pyrophosphate Sigma-Aldrich Cat# F6892 Farnesol Abcam Cat# ab142428 Geranylgeranyl pyrophosphate Sigma-Aldrich Cat# G6025 Geranylgeraniol Abcam Cat# ab142436 Gallein Santa Cruz Cat# sc-202631 Rho inhibitor, Rhosin Calbiochem Cat# 555460 Squalene Sigma-Aldrich Cat# S3626 Coenzyme Q9 Sigma-Aldrich Cat# 27597 Coenzyme Q10 Sigma-Aldrich Cat# C9538 Tamoxifen Sigma-Aldrich Cat# T5648 4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 D-glucose Sigma-Aldrich Cat# G7021 Oligomycin Adipogen Cat# 11342 Isoproterenol Sigma-Aldrich Cat# I5627 Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 FCCP Sigma-Aldrich Cat# C2920 Rotenone Sigma-Aldrich Cat# R8875 Antimycin A Sigma-Aldrich Cat# A8674 Sodium pyruvate Invitrogen Cat# 11360070 Seahorse XF Base Medium Agilent Cat# 102353 Complete Protease Inhibitors Roche Cat# 05056489001 Halt Phosphatase Inhibitors ThermoFischer Cat# 78426 Click-IT Geranylgeranyl Alcohol, Azide Invitrogen Cat# C10249 Biotin Alkyne Invitrogen Cat# B10185 Phalloidin-iFluor 488 Reagent Abcam Cat# ab176753 Critical Commercial Assays High Capacity cDNA RT kit Applied Biosystems Cat# 4368814 TruSeq RNA library prep kit Illumina Cat# RS-122-2301 DC Protein Assay Bio-Rad Cat# 5000111 Click-IT Protein Reaction Kit Invitrogen Cat# C10276 XFE96 FluxPak Agilent Cat# 102416-100 Glycerol reagent Sigma-Aldrich Cat# F6428 QBT fatty acid uptake assay kit Molecular Devices Cat# R6132 Luciferase reporter system Promega Cat# E1501 Autokit Total Ketone Bodies R1 Set Wako Chemicals Cat# 415-73301 Autokit Total Ketone Bodies R2 Set Wako Chemicals Cat# 413-73601 Deposited Data RNA sequencing data for clinical transcriptome study European Nucleotide Archive PRJEB23275 Experimental Models: Cell Lines immortalized brown adipocytes Christian Wolfrum Klein et al., 2002 hMADS cells Ez-Zoubir Amri Elabd et al., 2009 (Continued on next page) e2 Cell Metabolism 29, 1–16.e1–e8, April 2, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Organisms/Strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 Ucp1-CreERT2 x LoxP-Red mice Christian Wolfrum Rosenwald et al., 2013 Pggt1bfl/fl mice Martin Bergo Sjogren et al., 2007 Adip-CreERT2 mice Christian Wolfrum Rosenwald et al., 2013 Oligonucleotides qPCR primers Microsynth Listed in Table S4 siRNAs Microsynth Listed in Table S3 Software and Algorithms Harmony - Operetta software Perkin Elmer version 3.5 ImageJ NIH, USA version 1.50b Phenomaster software TSE systems version 5.6.5 Wave - XF96 software Agilent version 2.3.0.19 ImageQuant LAS 4000 GE Healthcare version 1.1 GraphPad Prism 7 GraphPad software version 7.03 ViiA7 software Applied Biosystems version 1.2.3 Gen5 BioTek version 1.10

    RNA Sequencing:

    Article Title: Inhibition of Mevalonate Pathway Prevents Adipocyte Browning in Mice and Men by Affecting Protein Prenylation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies UCP1 Thermo Fisher Cat# PA1-24894; RRID: AB_2241459 HSP90 Cell Signaling Cat# 4877; RRID: AB_2233307 g-TUBULIN Sigma-Aldrich Cat# T-5326; RRID: AB_532292 HSL Cell Signaling Cat# 4107; RRID: AB_2296900 phospho-HSL (Ser660) Cell Signaling Cat# 4126; RRID: AB_490997 AKT Cell Signaling Cat# 9272; RRID: AB_329827 phospho-AKT (Thr308) Cell Signaling Cat# 13038; RRID: AB_2629447 CREB Cell Signaling Cat# 9197; RRID: AB_331277 phospho-CREB (Ser133) Cell Signaling Cat# 9198; RRID: AB_2561044 PGGT1B Abcam Cat# ab122122; RRID: AB_11129941 RABGGTB Abcam Cat# ab187717; RRID:AB_2751007 GAPDH Cell Signaling Cat# 5174; RRID: AB_10622025 cleaved Caspase 3 (Asp175) Cell Signaling Cat# 9661; RRID: AB_2341188 RHOA Cell Signaling Cat# 2117; RRID: AB_10693922 RAC1/2/3 Cell Signaling Cat# 2465; RRID: AB_10695732 CDC42 Cell Signaling Cat# 2466; RRID: AB_2078082 RAP1A/RAP1B Cell Signaling Cat# 4938; RRID: AB_2177112 Na+/K+ ATPase Abcam Cat# ab76020; RRID: AB_1310695 HMGCS2 Abcam Cat# ab137043; RRID: AB_2749817 YAP1/TAZ Cell Signaling Cat# 8418; RRID: AB_10950494 PPARg Cell Signaling Cat# 2443; RRID: AB_10694772 HMGCR Abcam Cat# ab174830; RRID: AB_2749818 anti-mouse HRP secondary Millipore Cat# 401253; RRID: AB_437779 anti-rabbit HRP secondary Millipore Cat# 401393; RRID: AB_10683386 Streptavidin-HRP Cell Signaling Cat# 3999; RRID: AB_10830897 Chemicals, Peptides, and Recombinant Proteins Hoechst 33342 Cell Signaling Cat# 4082 Bodipy 493/503 Invitrogen Cat# D3922 Syto60 Invitrogen Cat# S11342 3-Isobutyl-1-methylxanthine Sigma-Aldrich Cat# I5879 Dexamethasone Sigma-Aldrich Cat# D4902 Indomethacin Sigma-Aldrich Cat# I7378 Insulin Sigma-Aldrich Cat# I9278 Rosiglitazone Adipogen Cat# 71740 Triiodo-L-Thyronine (T3) Sigma-Aldrich Cat# T6397 CL-316,243 Sigma-Aldrich Cat# C5976 Collagen, Type I Sigma-Aldrich Cat# C3867 DMEM, low glucose Lonza Cat# BE12-707F Ham’s F12 medium Lonza Cat# BE12-615F Penicillin-Streptomycin Gibco Cat# 15070063 DMEM, high glucose Gibco Cat# 41965062 L-glutamine Gibco Cat# 25030-024 Trizol reagent Invitrogen Cat# 15596026 DNase I (RNase-free) NEB Cat# M0303 Simvastatin Sigma-Aldrich Cat# S6196 (Continued on next page) Cell Metabolism 29, 1–16.e1–e8, April 2, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Fluvastatin Sigma-Aldrich Cat# SML0038 Atorvastatin Sigma-Aldrich Cat# PZ0001 Rosuvastatin Sigma-Aldrich Cat# SML1264 Pravastatin Sigma-Aldrich Cat# P4498 Cerivastatin Sigma-Aldrich Cat# SML0005 GGTI-298 Sigma-Aldrich Cat# G5169 FTI-277 Sigma-Aldrich Cat# F9803 Farnesyl pyrophosphate Sigma-Aldrich Cat# F6892 Farnesol Abcam Cat# ab142428 Geranylgeranyl pyrophosphate Sigma-Aldrich Cat# G6025 Geranylgeraniol Abcam Cat# ab142436 Gallein Santa Cruz Cat# sc-202631 Rho inhibitor, Rhosin Calbiochem Cat# 555460 Squalene Sigma-Aldrich Cat# S3626 Coenzyme Q9 Sigma-Aldrich Cat# 27597 Coenzyme Q10 Sigma-Aldrich Cat# C9538 Tamoxifen Sigma-Aldrich Cat# T5648 4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 D-glucose Sigma-Aldrich Cat# G7021 Oligomycin Adipogen Cat# 11342 Isoproterenol Sigma-Aldrich Cat# I5627 Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 FCCP Sigma-Aldrich Cat# C2920 Rotenone Sigma-Aldrich Cat# R8875 Antimycin A Sigma-Aldrich Cat# A8674 Sodium pyruvate Invitrogen Cat# 11360070 Seahorse XF Base Medium Agilent Cat# 102353 Complete Protease Inhibitors Roche Cat# 05056489001 Halt Phosphatase Inhibitors ThermoFischer Cat# 78426 Click-IT Geranylgeranyl Alcohol, Azide Invitrogen Cat# C10249 Biotin Alkyne Invitrogen Cat# B10185 Phalloidin-iFluor 488 Reagent Abcam Cat# ab176753 Critical Commercial Assays High Capacity cDNA RT kit Applied Biosystems Cat# 4368814 TruSeq RNA library prep kit Illumina Cat# RS-122-2301 DC Protein Assay Bio-Rad Cat# 5000111 Click-IT Protein Reaction Kit Invitrogen Cat# C10276 XFE96 FluxPak Agilent Cat# 102416-100 Glycerol reagent Sigma-Aldrich Cat# F6428 QBT fatty acid uptake assay kit Molecular Devices Cat# R6132 Luciferase reporter system Promega Cat# E1501 Autokit Total Ketone Bodies R1 Set Wako Chemicals Cat# 415-73301 Autokit Total Ketone Bodies R2 Set Wako Chemicals Cat# 413-73601 Deposited Data RNA sequencing data for clinical transcriptome study European Nucleotide Archive PRJEB23275 Experimental Models: Cell Lines immortalized brown adipocytes Christian Wolfrum Klein et al., 2002 hMADS cells Ez-Zoubir Amri Elabd et al., 2009 (Continued on next page) e2 Cell Metabolism 29, 1–16.e1–e8, April 2, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Organisms/Strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 Ucp1-CreERT2 x LoxP-Red mice Christian Wolfrum Rosenwald et al., 2013 Pggt1bfl/fl mice Martin Bergo Sjogren et al., 2007 Adip-CreERT2 mice Christian Wolfrum Rosenwald et al., 2013 Oligonucleotides qPCR primers Microsynth Listed in Table S4 siRNAs Microsynth Listed in Table S3 Software and Algorithms Harmony - Operetta software Perkin Elmer version 3.5 ImageJ NIH, USA version 1.50b Phenomaster software TSE systems version 5.6.5 Wave - XF96 software Agilent version 2.3.0.19 ImageQuant LAS 4000 GE Healthcare version 1.1 GraphPad Prism 7 GraphPad software version 7.03 ViiA7 software Applied Biosystems version 1.2.3 Gen5 BioTek version 1.10

    other:

    Article Title: Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes.
    Article Snippet: Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl mice GemPharmatech N/A Oligonucleotides qPCR primers Microsynth Listed in STAR Methods siRNAs Microsynth Listed in STAR Methods Recombinant DNA pLenti-MP2 plasmid Addgene Cat# 36097; RRID: Addgene_36097 Atp5k promoter sequences GenScript N/A pAAV-CA Addgene Cat# 69616; RRID: Addgene_69616 Software and algorithms Cellranger 10X Genomics V4.0.0 CellBender Fleming et al.63 V0.2.0 scDblFinder Pierre-Luc Germain64 V1.5.11 Seurat Hao et al.65 V4.0.4 SingleR Aran et al.66 V1.6.1 clusterProfiler Wu et al.67 V4.8.1 SUSHI Hatakeyama et al.68; Qi et al.69 N/A STAR Dobin et al.70 v2.7.4a (Continued on next page) e2 Cell Metabolism 36, 1–16.e1–e7, September 3, 2024



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